BP811ET. ADVANCED INSTRUMENTATION TECHNIQUES Books
All books are given below after syllabus
Unit-I
Nuclear Magnetic Resonance spectroscopy
Principles of H-NMR and C-NMR, chemical shift, factors affecting chemical shift, coupling
constant, Spin - spin coupling, relaxation, instrumentation and applications.
Mass Spectrometry- Principles, Fragmentation, Ionization techniques- Electron impact,
chemical ionization, MALDI, FAB, Analyzers -Time of flight and Quadrupole, instrumentation,
applications.
Unit-II
Thermal Methods of Analysis: Principles, instrumentation and applications of
Thermogravimetric Analysis (TGA), Differential Thermal Analysis (DTA), Differential
Scanning Calorimetry (DSC).
X-Ray Diffraction Methods: Origin of X-rays, basic aspects of crystals, X-ray Crystallography,
rotating crystal technique, single crystal diffraction, powder diffraction, structural elucidation
and applications.
Unit-III
Calibration and validation- as per ICH and USFDA guidelines.
Calibration of following Instruments: Electronic balance, UV-Visible spectrophotometer, IR
spectrophotometer, Fluorimeter, Flame Photometer, HPLC and GC.
Unit-IV
Radio immune assay: Importance, various components, Principle, different methods, Limitation
and Applications of Radio immunoassay.
Extraction techniques: General principle and procedure involved in the solid phase extraction
and liquid-liquid extraction.
Unit-V
Hyphenated techniques- LC-MS/MS, GC-MS/MS, HPTLC-MS.
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